mtb h37rv reference strain (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC mtb h37rv reference strain
Schematic of the experimental workflow for method validation using <t>H37Rv-spiked</t> dust samples

Mtb H37rv Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtb+h37rv+reference+strain/Mycobacterium+tuberculosis%3B+subsp%2E+tuberculosis/pmc12870882-49-1-10
Average 98 stars, based on 2908 article reviews

mtb h37rv reference strain - by Bioz Stars, 2026-09

98/100 stars

Images

1) Product Images from "Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust"

Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

Journal: BMC Infectious Diseases

doi: 10.1186/s12879-025-12332-0

Figure Legend Snippet: Schematic of the experimental workflow for method validation using H37Rv-spiked dust samples

Techniques Used: Biomarker Discovery

$Determination of optimal Percoll density gradient for H37Rv separation from dust. ( A , D) Photographs of discontinuous ( A ) 5-layer and ( D ) 3-layer Percoll density gradients. Methylene blue was added to select layers during gradient preparation for visual clarity. The distinct interphases after centrifugation are formed by intrinsic density differences. Pure H37Rv (acid-fast stained, red bacilli) and unprocessed dust particles were layered. The pink background in the MTB sample tubes originates from the suspension medium of the stained bacilli; the actual localization of MTB is indicated by the turbid, cloudy bands at the density interfaces. ( B , E ) Quantitative comparison of H37Rv DNA recovery efficiency across gradient fractions, as determined by qPCR. Data are presented as mean ± SD ( n = 3). Statistical significance was analyzed by one-way ANOVA for multi-group comparisons and unpaired t-test for pairwise comparisons (*** p < 0.001, **** p < 0.0001). ( C ) Representative microscopic fields (1000× magnification) of acid-fast stained bacilli recovered from each layer of the 5-layer gradient (scale bar: 100 μm)$
Figure Legend Snippet: Determination of optimal Percoll density gradient for H37Rv separation from dust. ( A , D) Photographs of discontinuous ( A ) 5-layer and ( D ) 3-layer Percoll density gradients. Methylene blue was added to select layers during gradient preparation for visual clarity. The distinct interphases after centrifugation are formed by intrinsic density differences. Pure H37Rv (acid-fast stained, red bacilli) and unprocessed dust particles were layered. The pink background in the MTB sample tubes originates from the suspension medium of the stained bacilli; the actual localization of MTB is indicated by the turbid, cloudy bands at the density interfaces. ( B , E ) Quantitative comparison of H37Rv DNA recovery efficiency across gradient fractions, as determined by qPCR. Data are presented as mean ± SD ( n = 3). Statistical significance was analyzed by one-way ANOVA for multi-group comparisons and unpaired t-test for pairwise comparisons (*** p < 0.001, **** p < 0.0001). ( C ) Representative microscopic fields (1000× magnification) of acid-fast stained bacilli recovered from each layer of the 5-layer gradient (scale bar: 100 μm)

Techniques Used: Centrifugation, Staining, Suspension, Comparison

Viability assessment of H37Rv recovered via Percoll separation. ( A-E ) Macroscopic appearance of H37Rv colonies on LJ medium after 4 weeks of incubation. Left panels: Recovery standard controls (direct inoculation of pure H37Rv suspensions without dust). Right panels: Colonies recovered via Percoll separation from H37Rv-spiked dust samples. Initial spiking concentrations: ( A ) 10⁰, ( B ) 10¹, ( C ) 10², ( D ) 10³, ( E ) 10⁴ CFU/mL. ( F ) Quantitative comparison of viable MTB concentrations (CFU/mL) between Percoll-separated samples (from dust matrix) and direct inoculation controls (pure H37Rv). Colony counts were converted to CFU/mL for analysis. Bars represent mean ± SD ( n = 3). Statistical significance was determined by a paired t-test (ns, not significant; ** p < 0.01)

Figure Legend Snippet: Viability assessment of H37Rv recovered via Percoll separation. ( A-E ) Macroscopic appearance of H37Rv colonies on LJ medium after 4 weeks of incubation. Left panels: Recovery standard controls (direct inoculation of pure H37Rv suspensions without dust). Right panels: Colonies recovered via Percoll separation from H37Rv-spiked dust samples. Initial spiking concentrations: ( A ) 10⁰, ( B ) 10¹, ( C ) 10², ( D ) 10³, ( E ) 10⁴ CFU/mL. ( F ) Quantitative comparison of viable MTB concentrations (CFU/mL) between Percoll-separated samples (from dust matrix) and direct inoculation controls (pure H37Rv). Colony counts were converted to CFU/mL for analysis. Bars represent mean ± SD ( n = 3). Statistical significance was determined by a paired t-test (ns, not significant; ** p < 0.01)

Techniques Used: Incubation, Comparison

Detection of MTB in air-conditioning dust from TB hospital wards using qPCR. ( A–D ) Representative qPCR amplification curves for MTB DNA detected in 25 clinical dust samples. Dashed lines: samples processed by Percoll separation. Solid lines: samples processed by direct DNA extraction. ( E ) qPCR amplification profiles for positive controls (H37Rv genomic DNA, red lines) and negative controls (no-template, green lines). ( F ) Examples of abnormal amplification curves obtained from the direct extraction method. ( G ) Violin plots comparing MTB DNA copy numbers between Percoll-based separation and direct extraction methods. Horizontal lines within violins represent the median and interquartile range. Statistical significance was determined by the Mann-Whitney U test (**** p < 0.0001)

Figure Legend Snippet: Detection of MTB in air-conditioning dust from TB hospital wards using qPCR. ( A–D ) Representative qPCR amplification curves for MTB DNA detected in 25 clinical dust samples. Dashed lines: samples processed by Percoll separation. Solid lines: samples processed by direct DNA extraction. ( E ) qPCR amplification profiles for positive controls (H37Rv genomic DNA, red lines) and negative controls (no-template, green lines). ( F ) Examples of abnormal amplification curves obtained from the direct extraction method. ( G ) Violin plots comparing MTB DNA copy numbers between Percoll-based separation and direct extraction methods. Horizontal lines within violins represent the median and interquartile range. Statistical significance was determined by the Mann-Whitney U test (**** p < 0.0001)

Techniques Used: Amplification, DNA Extraction, Extraction, MANN-WHITNEY

Positive Control:

Article Title: Increased detection of smear-negative pulmonary tuberculosis by GeneXpert MTB/RIF® assay after bleach concentration.
Article Snippet: .. The MTB H37Rv reference strain (American Type Culture Collection 27294) was processed with each run as a positive control. .. All positive cultures were confirmed for MTBC with SD Bioline MPT64TB Ag test (Standard Diagnostics, Yongin, South Korea).

Sterility:

Article Title: Rapid and Visual Differentiation of Mycobacterium tuberculosis From the Mycobacterium tuberculosis Complex Using Multiplex Loop-Mediated Isothermal Amplification Coupled With a Nanoparticle-Based Lateral Flow Biosensor
Article Snippet: .. The culture of the MTB H37Rv reference strain (ATCC 25177) was homogenized by using a multichannel rapid mixer (Zhu-hai Yinke Medical Engineering Co., Ltd., Guangdong, China) for 1 min in sterile distilled water (10 mg/ml). .. The bacterial suspension was used to prepare a series of 10-fold dilutions in sterile distilled water (10 –1 to 10 –8 ), and the dilutions were inoculated on a plate including Middlebrook 7H10 agar (Difco, Franklin Lakes, NJ, United States) and OADC reagents (oleic acid–albumin–dextrose–catalase; Becton, Dickinson and Company, Sparks, MD, United States).

Concentration Assay:

Article Title: Increase of CD4 + CD25 high FoxP3 + cells impairs in vitro human microbicidal activity against Mycobacterium tuberculosis during latent and acute pulmonary tuberculosis
Article Snippet: Blood samples were used for whole blood IFN-γ release assay (QuantiFERON TB Gold In-Tube, Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. .. The ATCC Mtb H37Rv reference strain (ATCC27294) and four clinical isolates with distinct minimal inhibitory concentration (MIC) to first-line anti-Mtb drugs and IS6110 RFLP electrophoretic band pattern [ ], stored at the Núcleo de Doenças Infecciosas (NDI)/UFES repository, were used in the present study ( and ). .. Peripheral blood samples were collected by venipuncture in K 3 -EDTA-treated tubes (BD Vacutainer Blood Collection Tube, Becton & Dickinson, USA).

Generated:

Article Title: Molecular diagnostics for drug-resistant tuberculosis: Can genetic tests replace conventional diagnostics as predictors of drug resistance and clinical outcome?
Article Snippet: .. Variants relative to the Mtb H37Rv reference strain (ATCC 27294) were identified automatically from generated PSQ pyrograms using IdentiFire software (Qiagen, Valencia, CA). ..

Article Title: Shedding light on the performance of a pyrosequencing assay for drug-resistant tuberculosis diagnosis
Article Snippet: .. Variants relative to the Mtb H37Rv reference strain (ATCC 27294) were identified automatically from generated PSQ pyrograms using IdentiFire software (Qiagen, Valencia, CA). ..

Software:

Suspension:

Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust.
Article Snippet: .. 2.2 H37Rv Bacterial Culture and Suspension Preparation The MTB H37Rv reference strain (ATCC27294) was obtained from the American Type Culture Collection (ATCC, USA). ..

Cell Culture:

Article Title: Mycobacterial EsxG·EsxH (TB9.8·TB10.4) peptides as a subunit vaccine to booster BCG vaccination in an experimental model of pulmonary Tuberculosis
Article Snippet: .. The Mtb H37Rv reference strain (ATCC 25618) and clinical isolate 09005186 were cultured in Middlebrook 7H9 broth (BD Biosciences, East Rutherford, New Jersey, USA) supplemented with oleic acid-albumin-dextrose-catalase (OADC) (BD Biosciences, East Rutherford, New Jersey, USA), 0.5% glycerol and 0.5% tyloxapol at 37 °C with gentle agitation (70 rpm) until reaching an optical density (OD) of 1.3 to H37Rv and 09005186, which correspond to the mid-long phase of bacteria measured at 600 nm. ..

Review

mtb h37rv reference strain (ATCC)

Structured Review

Images

1) Product Images from "Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust"

Related Articles

Positive Control:

Sterility:

Concentration Assay:

Generated:

Software:

Suspension:

Cell Culture:

Gentle:

Bacteria:

Similar Products

Image Search Results